Skills:
-Viral & Bacterial Vaccines: Production, safety testing and quality control.
-Cell culture Rabies vaccine: Production, safety testing and quality control of neural and cell culture vaccines.
-Cell culture:,propagation,preservation, tissue culture media preparation for virus isolation, vaccine.
Long term expet in all viral diagnostics, isolation, propagation, purification, concentration and preservation.
-Other immo-based methods including; ELISA, IFT, Agglutination, CIE, Immunoblot, Western-Bolt.
-Qualitative and quantitative PCR, R-T PCR molecular techniques used in virology such as viral load of HIV and HCV ( ROCHE AMPILICOR ), PCR-ELISA based quantitative detection.
-HBV Genotyping EIA Based Assay. ( new )
-Long term work experiences in research and routine laboratory diagnosis: mainly of medical viral disease and other microbial infections including: HIV; Viral Hepatitis A,B,C,D,E, &F, Polio, Rubrlla, Herpes, Rabies, -Avian Flu,CMV, EBV, RIFT VALLEY, West Nile, Mycobacteria,Tetanus And Vibrio.
-Quality control of lab reagents used in virology and bacteriology.
-Routine calibration, maitainance & Quality control of lab instruments used in virology and bacteriology
-Safety testing of biological products, such as Rabies, Hepatitis,Meningitis vaccines; anti-venom, anti-tetanus, anti-D, anti-human globulins and factor VIII.
-Long term practical instructor in medical microbiology for under & postgraduate medical laboratory and other medical students .
-Operation .Training, programming, maintenance, calibration of all types of manual ELISA Readers, Washers and Full automated ELISA.
-Development and optimization of qualitative & Quantitative ELISA for research and diagnostic purposes including, Class Specific qualitative ELISA for Rabies, Rift valley, West Nile Virus and tetanus.
-Cytokine ELISA based assay, IL-4, IL10, IL-18, IL-21 and IFγ. HBV, HEV, HEV-71.