-
US$10000
-
Duration: 1 Month
-
Delivery mode: Online
-
Group size: 11 - 20
-
Instruction language:
English,
Tamil
-
Certificate provided:
No
Welcome to our online course on Genetic Engineering! (Course price can be adjusted depending on the students count)
This course is designed to provide you with a comprehensive understanding of the fundamental concepts and techniques used in genetic engineering.
In Unit I, you will learn about the basics of recombinant DNA technology, including the manipulation of DNA using restriction and modification enzymes, design of linkers and adaptors, and characteristics of cloning and expression vectors. You will also gain an understanding of vectors for insect, yeast, and mammalian systems, as well as prokaryotic and eukaryotic host systems. Additionally, you will learn about the introduction of recombinant DNA into host cells and selection methods.
Unit II will focus on DNA libraries, including the construction of genomic and cDNA libraries, artificial chromosomes such as BACs and YACs, chromosomal walking, and screening of DNA libraries using nucleic acid probes and antisera.
In Unit III, you will learn about DNA sequencing and amplification techniques, including Maxam Gilbert’s and Sanger’s methods of DNA sequencing, inverse PCR, nested PCR, AFLP-PCR, allele-specific PCR, assembly PCR, asymmetric PCR, hot start PCR, colony PCR, single-cell PCR, real-time PCR/qPCR with SYBR green assay, Taqman assay, molecular beacons, and site-directed mutagenesis.
Unit IV will cover the organization and structure of genomes, genome sequencing methods such as conventional and shotgun genome sequencing methods and next-generation sequencing technologies. You will also learn about ordering the genome sequence, genetic maps and physical maps, STS content-based mapping, restriction enzyme fingerprinting, hybridization mapping, radiation hybrid maps, optical mapping, ORF finding, and functional annotation.
In Unit V, you will gain an understanding of the current status of genome sequencing projects, introduction to functional genomics, microarrays, serial analysis of gene expression (SAGE), subtractive hybridization, DIGE, TOGA, yeast two-hybrid system, comparative genomics, proteogenomics, web resources for genomics, and applications of genome analysis and genomics.
In addition to the comprehensive syllabus outlined above, this Genetic Engineering course will also include interactive sessions and assignments to enhance your learning experience. You will have access to course materials such as lecture notes, videos, and additional readings to supplement your understanding of the topics covered.
Throughout the course, you will have the opportunity to engage with your peers and instructors through online discussion forums and interactive sessions, where you can ask questions, share insights and collaborate on projects.
By the end of this course, you will have a thorough understanding of the basics of recombinant DNA technology, DNA libraries, sequencing and amplification of DNA, organization and structure of genomes, and current status of genome sequencing projects. You will also have gained practical experience in applying genetic engineering techniques to real-world problems.
Don't miss this opportunity to expand your knowledge in the field of genetic engineering. Enroll today and join our community of learners from around the world!