AuroSeqTech Bioinformatics Scientist
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Topics Covered:
Whole Genome Sequencing (Reference & De Novo Assembly)
RNA-Seq Data Analysis (Differential Expression, Annotation)
Metagenomics & Microbiome Analysis
GATK Variant Calling & Annotation
Organelle & Viral Genome Sequencing
Structural Bioinformatics & Computational Modeling

Modules
I Whole Genome Sequencing analysis of Bacteria (Reference based)
Quality filtering of raw reads (FastP, FastQC, MultiQC, Seqkit)
Contamination check (Kraken2)
Align reads to Reference genome (BWAMEM2, Samtools, sambamba)
Bam QC (Qualimap)
Detect variants (freebayes)
Variant Annotation (SnpEff, SnpSift)
Visualization of BAM (IGV)

II Whole Genome Sequencing analysis of Bacteria (de novo )
Quality filtering of raw reads (FastP, FastQC, MultiQC)
Contamination check (Kraken2)
Genome Assembly (Spades)
Assembly QC check (Quast)
Genome quality assessment (N50,L50 BUSCO)
Gene prediction (Prokka)
COG / CaZy analysis
BLASTN and BLASTP
Pathway Analysis
Genome Visualization
Secondary Metabolite Prediction
Genomics Island analysis
Anti microbial resistance analysis
Gene prediction and functional annotation

III Whole Transcriptome Sequencing (RNASeq Reference based)
Quality filtering of raw reads (FastP, FastQC, MultiQC, Seqkit)
Align reads to Reference genome( HiSat2)
Quantification of gene expression (FeatureCounts Subread)
Differential gene expression analysis ( DeSeq2)
Visualization: heatmaps, PCA, volcano plots
Pathway and Gene Enrichment Analysis ( ClusterProfier)
Gene Network analysis (STRING, CytoScape)
Coexpression network construction

IV Whole Transcriptome Sequencing (RNASeq de novo)
Quality filtering of raw reads (FastP, FastQC, MultiQC, Seqkit)
Transcriptome assembly
Unigene and isoform identification
Quantification of transcript expression
Differential gene expression analysis
Functional enrichment and pathway analysis
Noncoding RNA identification
Coexpression network construction
Visualization: heatmaps, PCA, volcano plots

V LongRead Sequencing (Galaxy cloud server)
Quality control and read correction
De novo (canu / hifiasm)
ploidy (genomescope 2.0)
Genome completeness (BUSCO)
Repeat Masking (RepeatModeller)
Gene Prediction (BREAKER 2)
Genome annotation

VI Metagenome Sequencing
Analyze metagenome using automated pipeline nextflow
Sequencing data processing and QC (FastP, FastQC, MultiQC, Seqkit)
Taxonomic profiling (Kraken2)
Functional annotation (KEGG, COG, eggNOGMapper)
Alpha and beta diversity analysis
Metagenomeassembled genome (MAG) construction
Strainlevel resolution
Resistome and virulome analysis

VII 16S rRNA Sequencing (Microbiome Analysis)
Raw read quality control (QIIME2 or Mothur)
OTU / ASV clustering
Taxonomic classification (Greengenes, SILVA)
Alpha and beta diversity analysis
Species level identification

VIII Structural Bioinformatics
Whether you’re a student, researcher, or professional looking to enhance your bioinformatics skills, I

IX Statistics analysis Support

X Research and Publication support

XI Epidemiology and Bioinformatics

Subjects

Bioinformatics Beginner-Expert
Biostatistics Expert
TCGA Expert
Next Generation Sequencing Beginner-Expert
Genome sequencing Expert
Structural Bioinformatics Beginner-Expert
Algorithms in Bioinformatics Expert
Biostatistics and Epidemiology Intermediate
Metagenomics Beginner-Expert
Advanced Bioinformatics Expert
Bioinformatics projects Beginner-Expert
Research and Publication Expert
Biological database development in bioinformatics Expert
Genome Analysis Expert

Experience

Bioinformatics Consultant (Sep, 2024–Present) at CMR-NIE Chennai India

Working on the ARCH Phase III project, a collaborative initiative between ICMR (India) and CDC(USA), focused on antimicrobial resistance in Enterobacterales from community and hospital settings. Performed bacterial whole genome sequencing analysis to detect resistance to cephalosporins,carbapenems, and colistin.Developed and automated pipelines for genome assembly, AMR gene detection
Scientific Advisor (Jan, 2024–Present) at AuroSeqTech Pondicherry

Research Guidance and Training support
AuroSeqTech Pondicherry
Scientist (Feb, 2022–May, 2024) at LifeCell International Private Limited

Whole Genome Analysis Projects Prokaryotic, Eukaryotic, Virus (reference /denovo), Genome Assembly - MaSurCa, RepeatMasking, BREAKER3, Genome completeness analysis (BUSCO)
● Transcriptome Analysis (Reference /denovo) STAR, HISAT2, TRINITY
● Circular RNA (DCC, CircExplorer2), LncRNAs (CPC2, CPAT tools, Lncipedia, RNACentral, and NONCODE)
● Co-expression Network Analysis
(gephi, Cytoscape) Protein-Protein Interaction Network analysis (Cytoscape-BINGO, ClueGo, MCODE)
● COVID-Seq (Variant analysis - nfcore/viralrecon)
● Organelle genome annotation (Mitocondria)
● Plasmid Genome Annotation
● Shotgun Metagenome (Standard and Binding) - nf-core MAG automated pipeline
● NCBI SRA data submission
● Team Lead & Handling clients
Scientist (Apr, 2021–Apr, 2022) at Nucleome Informatics, Hyderabad

Responsibilities and Project Handled
● Whole Genome Analysis Projects (reference /de novo)
● Exome GATK Pipeline, samtools, freebayes, SnpEff, ClinVar, dbSNP, gnomAD, COSMIC, TCGA
● Shotgun Metagenome (Standard/Binning)
● Amplicon (16S)
● De novo genome assembly (PacBio- Hybrid)
● Phylogenetic tree construction
● Biomarker Identification (Microarray & RNA seq)
● GBS (TASSEL)
● Team Lead & Handling clients

Education

Bioinformatics (Sep, 2014–Jan, 2022) from Pondicherry University, Puducherry
bioinformatics (Sep, 2014–Feb, 2022) from PONDICHEERY UNIVERSITY
Bioinformatics (Jun, 2004–Apr, 2006) from bharathidasan university

Fee details

₹500/hour (US$5.26/hour)

Courses offered

Whole de novo Genome Analysis of Bacteria
- ₹50000
- Duration: 1 Month
- Delivery mode: Online
- Group size: 11 - 20
- Instruction language: English
- Certificate provided: Yes
Module 1-Introduction to De Novo Assembly & Genomics
Module 2-Sample Preparation, Sequencing & QC
Module 3-De Novo Genome assembly
Module 4-Assembly Evaluation and Quality Assessment
Module 5- Genome Annotation with Prokka
Module 6- Functional Annotation & Pathway Analysis
Module 7-Comparative Genomics
Whole Shotgun Metagenomes
- ₹100000
- Duration: 1 Month
- Delivery mode: Online
- Group size: 6 - 10
- Instruction language: English
- Certificate provided: Yes
Bioinformatics Tools
Data cleaning and Pre-Processing
Quality Control – SeqKit, FastQC, Fastp, MultiQC
Read Trimming – Fastp
Contamination Check
Contamination Check – Kraken2
Genome assembly
Genome Assembly & QC – SPAdes, MEGAHIT, QUAST
Binning and Bin processing
Metabat2
Maxbin2
CONCOCT
Bin refinement
DAS tool
Evaluation of BIin quality
BUSCO /CheckM
Taxonomy Identification
GTDBK
Gene prediction
Prokka
Report
Decoding the Transcriptome: A Step-by-Step Guide for Beginners
- ₹100000
- Duration: 1 Month
- Delivery mode: Online
- Group size: 11 - 20
- Instruction language: English
- Certificate provided: Yes
Data cleaning and Preprocessing
Genome alignment
featurecount generation
Differential gene expression analysis
Gene enrichment analysis
Pathway analysis
Gene Network analysis
Bioinformatics Data analysis
- ₹10000
- Duration: 2 Weeks
- Delivery mode: Online
- Group size: Individual
- Instruction language: English
- Certificate provided: No
I you are looking for Bioinformatics data analysis like
Next-Generation Sequencing (NGS) Analysis
Whole Genome Analysis (Reference / de novo)
Transcriptome Analysis (Reference / de novo)
Long read sequencing data analysis
Shotgun Metagenomics, 16S, ITS, Metabarcoding
Plasmid, Phage & Viral Genomics
Organelle genomics (Mitochondria / Chloroplast)
Molecular Phylogenetics
Structural Bioinformatics
Please contact me
Data analysis price negotiable